Title
No effect of C-reactive protein on early atherosclerosis development in apolipoprotein E*3-Leiden/human C-reactive protein transgenic mice
Author
Trion, A.
de Maat, M.P.M.
Jukema, J.W.
van der Laarse, A.
Maas, M.C.
Offerman, E.H.
Havekes, L.M.
Szalai, A.J.
Princen, H.M.G.
Emeis, J.J.
TNO Kwaliteit van Leven
Publication year
2005
Abstract
Objective - C-reactive protein (CRP) has been associated with risk of cardiovascular disease. It is not clear whether CRP is causally involved in the development of atherosclerosis. Mouse CRP is not expressed at high levels under normal conditions and increases in concentration only several-fold during an acute phase response. Because the dynamic range of human CRP is much larger, apolipoprotein E*3-Leiden (E3L) transgenic mice carrying the human CRP gene offer a unique model to study the role(s) of CRP in atherosclerosis development. Methods and Results - Atherosclerosis development was studied in 15 male and 15 female E3L/CRP mice; E3L transgenic littermates were used as controls. The mice were fed a hypercholesterolemic diet to induce atherosclerosis development. Cholesterol exposure did not differ between E3L/CRP and E3L mice. Plasma CRP levels were on average 10.2±6.5 mg/L in male E3L/CRP mice, 0.2±0.1 mg/L in female E3L/CRP mice, and undetectable in E3L mice. Quantification of atherosclerosis showed that lesion area in E3L/CRP mice was not different from that in E3L mice. Conclusion - This study demonstrates that mildly elevated levels of CRP in plasma do not contribute to the development of early atherosclerosis in hypercholesterolemic E3L/CRP mice. © 2005 American Heart Association, Inc. Chemicals / CAS: C reactive protein, 9007-41-4; cholesterol, 57-88-5; Apolipoprotein E3; Apolipoproteins E; Biological Markers; C-Reactive Protein, 9007-41-4; Cholesterol, 57-88-5
Subject
Biology
Biomedical Research
Atherosclerosis
C-reactive protein
Inflammation
Mouse
Transgene
Acute phase protein
Apolipoprotein E3 Leiden
Biological marker
C reactive protein
Cholesterol
Unclassified drug
Animal experiment
Animal model
Animal tissue
Atherogenesis
Blood sampling
Cardiovascular risk
Cholesterol blood level
Cholesterol diet
Controlled study
Enzyme linked immunosorbent assay
Experimental model
Histopathology
Hypercholesterolemia
Immunohistochemistry
Inflammation
Mouse strain
Nonhuman
Pathophysiology
Protein blood level
Protein expression
Protein function
Transgenic mouse
Animals
Apolipoprotein E3
Apolipoproteins E
Atherosclerosis
Biological Markers
Body Weight
C-Reactive Protein
Cholesterol
Early Diagnosis
Eating
Endothelium, Vascular
Female
Humans
Hypercholesterolemia
Male
Mice
Mice, Transgenic
Monocytes
Risk Factors
Severity of Illness Index
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DOI
https://doi.org/10.1161/01.atv.0000171992.36710.1e
TNO identifier
238640
ISSN
1079-5642
Source
Arteriosclerosis, Thrombosis, and Vascular Biology, 25 (8), 1635-1640
Document type
article