Title
Encephalitogenic and immunogenic potential of the stress protein αB-crystallin in Biozzi ABH (H-2A(g7)) mice
Author
Thoua, N.-M.
van Noort, J.M.
Baker, D.
Bose, A.
van Sechel, A.C.
van Stipdonk, M.J.B.
Travers, P.J.
Amor, S.
TNO Preventie en Gezondheid
Publication year
2000
Abstract
The stress protein αB-crystallin is an immunodominant antigen in multiple sclerosis (MS)-affected myelin for human T cells and is expressed at elevated levels in MS lesions. Using bovine αB-crystallin and synthetic peptides based on mouse αB-crystallin the ability of this stress protein to induce experimental allergic encephalomyelitis (EAE) was screened in Biozzi ABH (H-2A(g7)) mice. While whole αB-crystallin and the immunodominant T cell epitopes (49-64, 73-88, 153-168) failed to induce disease the subdominant or cryptic epitope (1-16) was weakly encephalitogenic. The lack of encephalitogenicity of whole protein and dominant epitopes may be due to the low constitutive expression of αB-crystallin in the CNS combined with a state of peripheral tolerance suggested by the constitutive expression of αB-crystallin in secondary lymphoid tissues in ABH mice. Further evidence for a role of αB-crystallin in the progression of chronic relapsing neurological disease is suggested by the development of T cell responses to αB-crystallin during MOG-induced relapsing EAE as myelin damage accumulates. Together our data indicate that normal tolerising mechanisms in ABH mice prevent the induction of EAE by αB-crystallin while the subdominant or cryptic epitope is able to circumvent these mechanisms and contribute to pathogenic myelin-directed autoimmunity following T cell activation. (C) 2000 Elsevier Science, B.V. Chemicals/CAS: Crystallins; Epitopes
Subject
Health
αB-Crystallin
Experimental allergic encephalomyelitis
Multiple sclerosis
Peptide epitopes, tolerance
Alpha crystallin
Antigen
Beta crystallin
Epitope
Myelin
Allergic encephalomyelitis
Animal cell
Animal experiment
Animal model
Animal tissue
Autoimmunity
Immunological tolerance
Lymphoid tissue
Multiple sclerosis
Nonhuman
Protein expression
T lymphocyte activation
Animals
Cattle
Crystallins
Encephalitis
Epitopes
Lymphoid Tissue
Mice
Mice, Mutant Strains
Models, Chemical
Molecular Conformation
Peptide Mapping
T-Lymphocytes
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DOI
https://doi.org/10.1016/s0165-5728(99)00246-5
TNO identifier
235519
ISSN
0165-5728
Source
Journal of Neuroimmunology, 104 (1), 47-57
Document type
article