Print Email Facebook Twitter Ecology of the microbiome of the infected root canal system: A comparison between apical and coronal root segments Title Ecology of the microbiome of the infected root canal system: A comparison between apical and coronal root segments Author Özok, A.R. Persoon, I.F. Huse, S.M. Keijser, B.J.F. Wesselink, P.R. Crielaard, W. Zaura, E. Publication year 2012 Abstract Aim To evaluate the microbial ecology of the coronal and apical segments of infected root canal systems using a complete sampling technique and next-generation sequencing. Methodology The roots of 23 extracted teeth with apical periodontitis were sectioned in half, horizontally, and cryo-pulverized. Bacterial communities were profiled usring tagged 454 pyrosequencing of the 16S rDNA hypervariable V5-V6 region. Results The sequences were classified into 606 taxa (species or higher taxon), representing 24 bacterial phyla or candidate divisions and one archaeal phylum. Proteobacteria were more abundant in the apical samples (P<0.05), whilst Actinobacteria were in significantly higher proportions in the coronal samples. The apical samples harboured statistically significantly more taxa than the coronal samples (P=0.01) and showed a higher microbial diversity. Several taxa belonging to fastidious obligate anaerobes were significantly more abundant in the apical segments of the roots compared with their coronal counterparts. Conclusions Endodontic infections are more complex than reported previously. The apical part of the root canal system drives the selection of a more diverse and more anaerobic community than the coronal part. The presence of a distinct ecological niche in the apical region explains the difficulty of eradication of the infection and emphasizes the need for new treatment approaches to be developed. © 2012 International Endodontic Journal. Subject LifeMSB - Microbiology and Systems BiologyEELS - Earth, Environmental and Life SciencesHealthy for LifeBiologyHealthy LivingApicalApical periodontitisCoronalCryo-pulverizationMicrobiomePyrosequencing To reference this document use: http://resolver.tudelft.nl/uuid:3013ea34-6da7-4221-83d2-3c120a59078e DOI https://doi.org/10.1111/j.1365-2591.2011.02006.x TNO identifier 460434 ISSN 0143-2885 Source International Endodontic Journal, 45 (6), 530-541 Document type article Files To receive the publication files, please send an e-mail request to TNO Library.