Rapid and generic identification of respiratory viruses with mass spectrometry - Poster
de Jong, A.L.
van der Plas, J.
Tested respiratory viruses: influenza A virus, respiratory syncytial virus (RSV) and human metapneumovirus (hMPV), were successfully identified directly from crude cell cultures using liquid chromatography-tandem MS (LC-MS/MS). The identification was preceded with the simple, generic and fast sample preparation method. The determined identification limit was only one order of magnitude higher than required for identification from a clinical specimen. Because identification is based on amino acid sequences deposited in GenBank, other viruses are likely to be identified using the developed method. Further improvement of the sample preparation method, may soon bridge the gap between research and clinical diagnostics. We conclude that MS-based techniques have the potential to become a complementary method to PCR in viral diagnostics.
To reference this document use:
CBRN - CBRN Protection
TS - Technical Sciences
Amino acid sequence
Influenza virus A
Matrix assisted laser desorption ionization time of flight mass spectrometry
Respiratory syncytial pneumovirus
Tandem mass spectrometry
Influenza A virus
Respiratory syncytial virus
NVMM 2014, Arnhem, Nederland, 15-16 april 2014