Title
Rapid and generic identification of respiratory viruses with mass spectrometry - Poster
Author
Majchrzykiewicz-Koehorst, J.A.
Heikens, E.
Trip, H.
Hulst, A.G.
de Jong, A.L.
Viveen, M.C.
Sedee, N.J.A.
van der Plas, J.
Coenjaerts, F.E.J.
Paauw, A.
Publication year
2014
Abstract
Tested respiratory viruses: influenza A virus, respiratory syncytial virus (RSV) and human metapneumovirus (hMPV), were successfully identified directly from crude cell cultures using liquid chromatography-tandem MS (LC-MS/MS). The identification was preceded with the simple, generic and fast sample preparation method. The determined identification limit was only one order of magnitude higher than required for identification from a clinical specimen. Because identification is based on amino acid sequences deposited in GenBank, other viruses are likely to be identified using the developed method. Further improvement of the sample preparation method, may soon bridge the gap between research and clinical diagnostics. We conclude that MS-based techniques have the potential to become a complementary method to PCR in viral diagnostics.
Subject
Life
CBRN - CBRN Protection
TS - Technical Sciences
Biology
Identification
Influenza virus
LC-MS/MS
MALDI-TOF MS
Mass spectrometry
Virological diagnosis
virus protein
Amino acid sequence
Controlled study
Influenza virus A
Intermethod comparison
Liquid chromatography
Matrix assisted laser desorption ionization time of flight mass spectrometry
Metapneumovirus
Nonhuman
Nucleotide sequence
Process development
Reliability
Respiratory syncytial pneumovirus
Respiratory virus
RNA sequence
Sensitivity analysis
Sequence analysis
Tandem mass spectrometry
Virus identification
Virus strain
Bacteria (microorganisms)
Human metapneumovirus
Influenza A virus
Orthomyxoviridae
Respiratory syncytial virus
To reference this document use:
http://resolver.tudelft.nl/uuid:2f18903a-2092-4da3-bb77-1a9d468b647f
TNO identifier
531255
Source
NVMM 2014, Arnhem, Nederland, 15-16 april 2014
Document type
conference paper