Title
High-throughput analysis of the impact of antibiotics on the human intestinal microbiota composition
Author
Ladirat, S.E.
Schols, H.A.
Nauta, A.
Schoterman, M.H.C.
Keijser, B.J.F.
Montijn, R.C.
Gruppen, H.
Schuren, F.H.J.
Publication year
2013
Abstract
Antibiotic treatments can lead to a disruption of the human microbiota. In this in-vitro study, the impact of antibiotics on adult intestinal microbiota was monitored in a new high-throughput approach: a fermentation screening-platform was coupled with a phylogenetic microarray analysis (Intestinal-chip). Fecal inoculum from healthy adults was exposed in a fermentation screening-platform to seven widely-used antibiotics during 24. h in-vitro fermentation and the microbiota composition was subsequently determined with the Intestinal-chip.Phylogenetic microarray analysis was first verified to be reliable with respect to variations in the total number of bacteria and presence of dead (or inactive) cells. Intestinal-chip analysis was then used to identify and compare shifts in the intestinal microbial composition after exposure to low and high dose (1μgml-1 and 10μgml-1) antibiotics. Observed shifts on family, genus and species level were both antibiotic and dose dependent. Stronger changes in microbiota composition were observed with higher doses. Shifts mainly concerned the bacterial groups Bacteroides, Bifidobacterium, Clostridium, Enterobacteriaceae, and Lactobacillus. Within bacterial groups, specific antibiotics were shown to differentially impact related species.The combination of the in-vitro fermentation screening platform with the phylogenetic microarray read-outs has shown to be reliable to simultaneously analyze the effects of several antibiotics on intestinal microbiota. © 2012 Elsevier B.V.
Subject
Life
MSB - Microbiology and Systems Biology
EELS - Earth, Environmental and Life Sciences
Biomedical Innovation
Biology
Healthy Living
Antibiotics
In-vitro fermentation screening-platform
Intestinal microbiota
Phylogenetic microarray
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http://resolver.tudelft.nl/uuid:2c603c76-fafd-4198-a5bd-6d70cf543e34
DOI
https://doi.org/10.1016/j.mimet.2012.12.011
TNO identifier
470334
ISSN
0167-7012
Source
Journal of Microbiological Methods, 92 (3), 387-397
Document type
article