Title
Study of the glucoamylase promoter in Aspergillus niger using green fluorescent protein
Author
Santerre Henriksen, A.L.
Even, S.
Müller, C.
Punt, P.J.
van den Hondel, C.A.M.J.J.
Nielsen, J.
Centraal Instituut voor Voedingsonderzoek TNO
Publication year
1999
Abstract
An Aspergillus niger strain expressing a red-shifted green fluorescent protein (GFP) in the cytoplasm under the control of the glucoamylase promoter (PglaA) was characterized with respect to its physiology and morphology. Although xylose acted as a repressor carbon source during batch cultivations, PglaA-driven GFP expression by the glucoamylase promoter could be demonstrated in xylose-limited continuous cultures. In these cultivations, the xylose concentration was therefore too low to cause repression. Transient experiments initiated with a maltose pulse did not further induce red-shifted GFP production in xylose-limited continuous cultures. Maltose induction under conditions of xylose repression was microscopically observed and quantified in a flow-through chamber. Red-shifted GFP was first produced after 5 h induction. Finally the strain was characterized in glucose-limited continuous cultures, and here the area of the mycelium stained with cytoplasmic GFP increased with increasing specific growth rate, indicating that GFP can be used as a marker of cellular activity in this type of cultivation.
Subject
Nutrition
Aspergillus niger
Chemostat
Glucoamylase promoter
Green fluorescent protein
Glucan 1,4 alpha glucosidase
Green fluorescent protein
Maltose
Xylose
Aspergillus niger
Continuous culture
Fungus culture
Growth rate
Mycelium
Nonhuman
Priority journal
Aspergillus niger
Genes, Reporter
Glucan 1,4-alpha-Glucosidase
Green Fluorescent Proteins
Luminescent Proteins
Promoter Regions (Genetics)
Aspergillus niger
Fungi
To reference this document use:
http://resolver.tudelft.nl/uuid:287aafe7-c707-437d-8c25-dc587153da0b
TNO identifier
234971
ISSN
1350-0872
Source
Microbiology, 145 (3), 729-734
Document type
article