Title
Interlaboratory evaluation of a cow's milk allergy mouse model to assess the allergenicity of hydrolysed cow's milk based infant formulas
Author
van Esch, B.C.A.M.
van Bilsen, J.H.M.
Jeurink, P.V.
Garssen, J.
Penninks, A.H.
Smit, J.J.
Pieters, R.H.H.
Knippels, L.M.J.
Publication year
2013
Abstract
This study describes two phases of a multi-phase project aiming to validate a mouse model for cow's milk allergy to assess the potential allergenicity of hydrolysed cow's milk based infant formulas (claim support EC-directive 2006/141/E). The transferability and the discriminatory power of this model was evaluated in 4 research centers. Mice were sensitized by oral gavage with whey or extensively hydrolysed whey (eWH) using cholera toxin as an adjuvant. Whey-specific antibodies, mMCP-1 levels, anaphylactic shock symptoms, body temperature and the acute allergic skin response were determined upon whey challenge. In phases I and II, all 4 centers detected elevated levels of whey-specific IgE/IgG1 in whey sensitized animals. Elevated levels of mMCP-1, anaphylactic symptoms, body temperature drop and acute allergic skin response were scored upon whey challenge in 3 out of 4 research centers. In contrast, none of the evaluated parameters were elevated in eWH orally exposed groups. The cow's milk allergy mouse model is capable to distinguish the sensitizing capacity of complete or hydrolysed cow's milk protein. The model uses straightforward parameters relevant to food allergic responses and can be effectively transferred between different laboratories. We propose this mouse model as a new strategy for the screening of new hypoallergenic cow's milk formulas. © 2013 Elsevier Ireland Ltd.
Subject
Life Triskelion BV
QS - Quality & Safety RAPID - Risk Analysis for Products in Development
EELS - Earth, Environmental and Life Sciences TNO Bedrijven
Food and Nutrition
Biology
Healthy Living
Allergenicity
Cow's milk allergy
Hydrolysed infant formulas
Mouse model
Safety
Sensitization
To reference this document use:
http://resolver.tudelft.nl/uuid:2817700a-457e-46f5-b274-03fdbb9c829a
DOI
https://doi.org/10.1016/j.toxlet.2013.04.008
TNO identifier
472354
ISSN
0378-4274
Source
Toxicology Letters, 220 (1), 95-102
Document type
article