Title
Efficient screening methods for glucosyltransferase genes in Lactobacillus strains
Author
Kralj, S.
Geel van - Schutten, G.H.
van der Maarel, M.J.E.C.
Dijkhuizen, L.
Publication year
2003
Abstract
Limited information is available about homopolysaccharide synthesis in the genus Lactobacillus. Using efficient screening techniques, extracellular glucosyltransferase (GTF) enzyme activity, resulting in α-glucan synthesis from sucrose, was detected in various lactobacilli. PCR with degenerate primers based on homologous boxes of known glucosyltransferase (gtf) genes of lactic acid bacteria strains allowed cloning of fragments of 10 putative gtf genes from eight different glucan producing Lactobacillus strains (five Lactobacillus reuteri strains, one Lactobacillus fermentum strain, one Lactobacillus sake strain and one Lactobacillus parabuchneri strain). Sequence analysis revealed that these lactobacilli possess a large variation of (putative) gtf genes, similar to what has been observed for Leuconostoc and Streptococcus strains. Homologs of GTFA of Lb. reuteri 121 (synthesizing reuteran, a unique glucan with α(1 → 4) and α-(1 → 6) glycosidic bonds) (Kralj et al., 2002) were found in three of the four other Lb. reuteri strains tested. The other Lactobacillus GTF fragments showed the highest similarity with GTF enzymes of Leuconostoc spp.
Subject
Nutrition
Food technology
Glucan
Glucansucrase
Glucosyltransferase
Lactobacillus
Polysacch aride
Sucrose
Bacteria
Chemical bonds
Genes
Sugar (sucrose)
Synthesis (chemical)
Screening techniques
Enzymes
glucan
glucosyltransferase
lactic acid
polysaccharide
sucrose
bacterial strain
carbohydrate synthesis
cloning
conference paper
controlled study
enzyme activity
gene identification
genetic variability
glycosidation
Lactobacillus
Lactobacillus fermentum
Lactobacillus reuteri
Lactobacillus sakei
Leuconostoc
nonhuman
nucleotide sequence
polymerase chain reaction
screening test
sequence analysis
Streptococcus
Bacteria (microorganisms)
Lactobacillus
Lactobacillus fermentum
Lactobacillus parabuchneri
Lactobacillus reuteri
Lactobacillus sakei
Leuconostoc
Prokaryota
Streptococcus
To reference this document use:
http://resolver.tudelft.nl/uuid:27aa2f78-626d-4eae-a742-8c43c31abb48
DOI
https://doi.org/10.1080/10242420310001618519
TNO identifier
237207
ISSN
1024-2422
Source
Biocatalysis and Biotransformation, 21 (4-5), 181-187
Document type
article