Absence of an inhibitory effect of a vegetables-fruit mixture on the initiation and promotion phases of azoxymethane-induced colorectal carcinogenesis in rats fed low- or high-fat diets

Absence of an inhibitory effect of a vegetables-fruit mixture on the initiation and promotion phases of azoxymethane-induced colorectal carcinogenesis in rats fed low- or high-fat diets

Rijnkels, J.M.
Hollanders, V.M.H.
Woutersen, R.A.
Koeman, J.H.
Alink, G.M.
Centraal Instituut voor Voedingsonderzoek TNO

1998

The potential inhibitory effects of a vegetables-fruit mixture on the initiation and promotion phases of azoxymethane-induced colorectal carcinogenesis were examined in rats fed low- or high-fat diets. Rats were fed low-fat diets (20 energy percent, Diets A and B) or high-fat diets (40 energy percent, Diets C and D), supplemented with a vegetables-fruit mixture (19.5% wt/wt, Diets B and D) or unsupplemented (Diets A and C) for 36 weeks. After the animals were maintained on the respective diets for four weeks, they were given three weekly injections of azoxymethane at 15 mg/kg body wt sc. Eight weeks after the start of the study, animals maintained on Diet A were switched to Diet B or C or maintained on the same diet. Animals maintained on Diet B or D were switched to Diet A or C, respectively. Furthermore, animals maintained on Diet C were switched to Diet A or D or maintained on the same diet. Multiplicity of colorectal tumors did not differ between groups fed a vegetables-fruit mixture during the initiation or the promotion phase (Group B → A vs. Group A → B; Group D → C vs. Group C → D). However, multiplicity was significantly lower in animals fed low-fat diets than in animals fed high-fat diets in combination with a vegetables- fruit mixture (Group A → B/B → A vs. Group C → D/D → C). Furthermore, multiplicity was significantly increased in groups fed a high-fat diet during the promotion phase only in comparison with animals fed a low-fat diet during the whole experiment (Group A → C vs. Group A → A). No other differences in multiplicity or tumor incidences were observed among the eight experimental groups.

Nutrition
Azoxymethane
Animal experiment
Animal model
Animal tissue
Article
Carcinogenesis
Colorectal cancer
Controlled study
Dietary intake
Fruit
Lipid diet
Low fat diet
Male
Nonhuman
Rat
Tumor promotion
Tumor volume
Vegetable
Adenocarcinoma
Adenoma
Animals
Azoxymethane
Carcinogens
Colorectal Neoplasms
Diet
Diet, Fat-Restricted
Dietary Fats
Fruit
Male
Rats
Rats, Inbred F344
Vegetables

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Nutrition and Cancer, 30 (2), 124-129

article