Title
Immunological characterization of plasminogen activator activities in human tissues and body fluids
Author
Rijken, D.C.
Wijngaards, G.
Welbergen, J.
Gaubius instituut TNO
Publication year
1981
Abstract
Human plasminogen activators were compared immunologically in both a double-diffusion technique and quenching experiments on the fibrinolytic activities of the activators. Antisera against HMW and LMW urokinase and an antiserum against highly purified tissue plasminogen activator from human uterus were used. It was found that uterine tissue plasminogen activator and urokinase are two immunologically distinct plasminogen activators. The occurrence of the two kinds of plasminogen activators in human tissues and body fluids was studied on the basis of the quenching of the activities by antibodies. In tissue extracts, mainly tissue plasminogen activator was found. Seminal plasma exhibited a high plasminogen activator activity, consisting of both urokinase and tissue plasminogen activator-related activators. Urine contained a small amount of tissue plasminogen activator-related in addition to urokinase. The low plasminogen activator activities of saliva and tears were completely attributed to activators related to tissue plasminogen activator. Chemicals/CAS: plasminogen activator, 9039-53-6; Immune Sera; Plasminogen Activators, EC 3.4.21.-; Plasminogen, 9001-91-6; Urinary Plasminogen Activator, EC 3.4.21.73
Subject
Biology
Animal
Body Fluids
Comparative Study
Female
Human
Immune Sera
Immunodiffusion
Male
Plasminogen
Plasminogen Activators
Rabbits
Semen
Tissue Distribution
Urinary Plasminogen Activator
Uterus
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TNO identifier
229015
ISSN
0022-2143
Source
Journal of Laboratory and Clinical Medicine, 97 (4), 477-486
Document type
article