Title
Simultaneous measurement of foveal spectral reflectance and cone-photoreceptor directionality
Author
Zagers, N.P.A.
van de Kraats, J.
Berendschot, T.T.J.M.
van Norren, D.
Publication year
2002
Abstract
An instrument for simultaneous measurement of foveal spectral reflectance and cone-photoreceptor directionality is described. The key element is an imaging spectrograph (spectral range of 420–790 nm) with its entrance slit conjugate to the pupil plane of a human eye. A 1.9-deg spot on the retina is sampled in 1 s. Video observation of the retina and the pupil facilitates proper alignment. Measurements were performed on 21 healthy subjects. Model analysis of the spectra provided densities of photostable ocular absorbers. As an example, macular pigment and melanin are discussed in more detail. Spatial profiles exhibited the optical Stiles–Crawford effect, reflecting cone-photoreceptor directionality. © 2002 Optical Society of America.
Subject
biological model
instrumentation
middle aged
physiology
radiation scattering
retina cone
retina fovea
Adult
Aged
Cones (Retina)
Female
Fovea Centralis
Humans
Male
Middle Aged
Models, Biological
Ophthalmology
Pupil
Scattering, Radiation
Imaging techniques
Light absorption
Light reflection
Spectrographs
Spectrum analysis
Video recording
Cone-photoreceptors
Image analysis
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DOI
https://doi.org/10.1364/ao.41.004686
TNO identifier
953593
ISSN
1559-128X
Source
Applied Optics, 41 (41), 4686-4696
Document type
article