Print Email Facebook Twitter Plasma metabolomics and proteomics profiling after a postprandial challenge reveal subtle diet effects on human metabolic status Title Plasma metabolomics and proteomics profiling after a postprandial challenge reveal subtle diet effects on human metabolic status Author Pellis, L. van Erk, M.J. van Ommen, B. Bakker, G.C.M. Hendriks, H.F.J. Cnubben, N.H.P. Kleemann, R. van Someren, E.P. Bobeldijk, I. Rubingh, C.M. Wopereis, S. Publication year 2012 Abstract We introduce the metabolomics and proteomics based Postprandial Challenge Test (PCT) to quantify the postprandial response of multiple metabolic processes in humans in a standardized manner. The PCT comprised consumption of a standardized 500 ml dairy shake containing respectively 59, 30 and 12 energy percent lipids, carbohydrates and protein. During a 6 h time course after PCT 145 plasma metabolites, 79 proteins and 7 clinical chemistry parameters were quantified. Multiple processes related to metabolism, oxidation and inflammation reacted to the PCT, as demonstrated by changes of 106 metabolites, 31 proteins and 5 clinical chemistry parameters. The PCT was applied in a dietary intervention study to evaluate if the PCT would reveal additional metabolic changes compared to non-perturbed conditions. The study consisted of a 5-week intervention with a supplement mix of anti-inflammatory compounds in a crossover design with 36 overweight subjects. Of the 231 quantified parameters, 31 had different responses over time between treated and control groups, revealing differences in amino acid metabolism, oxidative stress, inflammation and endocrine metabolism. The results showed that the acute, short term metabolic responses to the PCT were different in subjects on the supplement mix compared to the controls. The PCT provided additional metabolic changes related to the dietary intervention not observed in non-perturbed conditions. Thus, a metabolomics based quantification of a standardized perturbation of metabolic homeostasis is more informative on metabolic status and subtle health effects induced by (dietary) interventions than quantification of the homeostatic situation. © 2011 The Author(s). Subject Life TriskelionMSB - Microbiology and Systems Biology MHR - Metabolic Health Research PHS - Pharmacokinetics & Human Studies RAPID - Risk Analysis for Products in DevelopmentEELS - Earth, Environmental and Life Sciences TNO BedrijvenHealthy for LifeBiologyHealthy LivingMetabolic profilingPlasmaPostprandial challengeProteomic profiling To reference this document use: http://resolver.tudelft.nl/uuid:11affe6e-89dc-4ebe-a12b-1f626c9b5f2a DOI https://doi.org/10.1007/s11306-011-0320-5 TNO identifier 447248 ISSN 1573-3882 Source Metabolomics, 8 (2), 347-359 Document type article Files To receive the publication files, please send an e-mail request to TNO Library.