Title
Visualization of proteases within a complex sample following their selective retention on immobilized bacitracin, a peptide antibiotic
Author
van Noort, J.M.
van den Berg, P.
Mattern, I.E.
Medisch Biologisch Laboratorium TNO
Publication year
1991
Abstract
A method for the visualization of individual proteases within a complex biological sample is described. In a single chromatographic step, proteases can be separated from other biomolecules by selective binding to immobilized bacitracin, a peptide antibiotic. Following desorption, proteases may be separated by SDS-polyacrylamide gel electrophoresis. The application of this method is presented in the visualization of proteases secreted by the fungus Aspergillus niger.
Subject
Bacitracin
Proteinase
Aspergillus niger
Chromatography
Nonhuman
Polyacrylamide gel electrophoresis
Priority journal
Protein analysis
Protein electrophoresis
Aspergillus niger
Bacitracin
Electrophoresis, Polyacrylamide Gel
Endopeptidases
Peptides
Protein Binding
Sepharose
Aspergillus niger
Fungi
To reference this document use:
http://resolver.tudelft.nl/uuid:098e5e30-e832-4b3e-88da-aef9943c9eb9
TNO identifier
231618
ISSN
0003-2697
Source
Analytical Biochemistry, 198 (2), 385-390
Bibliographical note
Chemicals/CAS: Bacitracin, 1405-87-4; Endopeptidases, EC 3.4.-; Peptides; Sepharose, 9012-36-6
Document type
article