Print Email Facebook Twitter Visualization of proteases within a complex sample following their selective retention on immobilized bacitracin, a peptide antibiotic Title Visualization of proteases within a complex sample following their selective retention on immobilized bacitracin, a peptide antibiotic Author van Noort, J.M. van den Berg, P. Mattern, I.E. Medisch Biologisch Laboratorium TNO Publication year 1991 Abstract A method for the visualization of individual proteases within a complex biological sample is described. In a single chromatographic step, proteases can be separated from other biomolecules by selective binding to immobilized bacitracin, a peptide antibiotic. Following desorption, proteases may be separated by SDS-polyacrylamide gel electrophoresis. The application of this method is presented in the visualization of proteases secreted by the fungus Aspergillus niger. Subject BacitracinProteinaseAspergillus nigerChromatographyNonhumanPolyacrylamide gel electrophoresisPriority journalProtein analysisProtein electrophoresisAspergillus nigerBacitracinElectrophoresis, Polyacrylamide GelEndopeptidasesPeptidesProtein BindingSepharoseAspergillus nigerFungi To reference this document use: http://resolver.tudelft.nl/uuid:098e5e30-e832-4b3e-88da-aef9943c9eb9 TNO identifier 231618 ISSN 0003-2697 Source Analytical Biochemistry, 198 (2), 385-390 Bibliographical note Chemicals/CAS: Bacitracin, 1405-87-4; Endopeptidases, EC 3.4.-; Peptides; Sepharose, 9012-36-6 Document type article Files To receive the publication files, please send an e-mail request to TNO Library.