Title
Semi-targeted metabolomic approaches to validate potential markers of health for micronutrients: Analytical perspectives
Author
Bayle, M.L.
Wopereis, S.
Bouwman, J.
van Ommen, B.
Scalbert, A.
Pujos-Guillot, E.
Publication year
2012
Abstract
Recommended dietary allowances for micronutrients fluctuate noticeably within European Union countries. The Network of Excellence EURRECA (EURopean micronutrient RECommendations Aligned) aims at harmonising micronutrient intake recommendations through population groups. The lack of proper markers of status for some micronutrients limits progress in this area: metabolomics could help identifying such new markers. We developed an original metabolomic strategy in order to monitor the largest fraction of a list of >270 metabolites known to be influenced by the micronutrients of interest. To improve the coverage of these metabolites in plasma, a multi platform approach was performed using both liquid and gas chromatography coupled to mass spectrometry. A sample preparation protocol based on a three-step plasma fractionation has been set up, using both liquid and solid phase extractions. Four fractions were obtained containing respectively polar metabolites, neutral lipids, free fatty acids and polar lipids. Recoveries were determined using spiked plasma samples, and the advantages and drawbacks of the fractionation method compared to a commonly used single preparation step method were investigated in terms of metabolites detection and robustness. Fractionation improved coverage of the endogenous metabolome more than twice in terms of extracted features, allowing to identify 90 metabolites. © 2012 Springer Science+Business Media, LLC.
Subject
Life
MSB - Microbiology and Systems Biology
EELS - Earth, Environmental and Life Sciences
Biomedical Innovation
Biology
Healthy Living
Fractionation
GC-MS
Mass spectrometry
Metabolomics
Plasma
QTOF
UPLC
To reference this document use:
http://resolver.tudelft.nl/uuid:08dc9d36-a6d5-415f-879e-41267e594b63
DOI
https://doi.org/10.1007/s11306-012-0419-3
TNO identifier
465808
ISSN
1573-3882
Source
Metabolomics, 8 (6), 1114-1129
Document type
article