Print Email Facebook Twitter Semi-targeted metabolomic approaches to validate potential markers of health for micronutrients: Analytical perspectives Title Semi-targeted metabolomic approaches to validate potential markers of health for micronutrients: Analytical perspectives Author Bayle, M.L. Wopereis, S. Bouwman, J. van Ommen, B. Scalbert, A. Pujos-Guillot, E. Publication year 2012 Abstract Recommended dietary allowances for micronutrients fluctuate noticeably within European Union countries. The Network of Excellence EURRECA (EURopean micronutrient RECommendations Aligned) aims at harmonising micronutrient intake recommendations through population groups. The lack of proper markers of status for some micronutrients limits progress in this area: metabolomics could help identifying such new markers. We developed an original metabolomic strategy in order to monitor the largest fraction of a list of >270 metabolites known to be influenced by the micronutrients of interest. To improve the coverage of these metabolites in plasma, a multi platform approach was performed using both liquid and gas chromatography coupled to mass spectrometry. A sample preparation protocol based on a three-step plasma fractionation has been set up, using both liquid and solid phase extractions. Four fractions were obtained containing respectively polar metabolites, neutral lipids, free fatty acids and polar lipids. Recoveries were determined using spiked plasma samples, and the advantages and drawbacks of the fractionation method compared to a commonly used single preparation step method were investigated in terms of metabolites detection and robustness. Fractionation improved coverage of the endogenous metabolome more than twice in terms of extracted features, allowing to identify 90 metabolites. © 2012 Springer Science+Business Media, LLC. Subject LifeMSB - Microbiology and Systems BiologyEELS - Earth, Environmental and Life SciencesBiomedical InnovationBiologyHealthy LivingFractionationGC-MSMass spectrometryMetabolomicsPlasmaQTOFUPLC To reference this document use: http://resolver.tudelft.nl/uuid:08dc9d36-a6d5-415f-879e-41267e594b63 DOI https://doi.org/10.1007/s11306-012-0419-3 TNO identifier 465808 ISSN 1573-3882 Source Metabolomics, 8 (6), 1114-1129 Document type article Files To receive the publication files, please send an e-mail request to TNO Library.