Title
Ranking of 10 legumes according to the prevalence of sensitization as a parameter to characterize allergenic proteins
Author
Smits, M.
Verhoeckx, K.
Knulst, A.
Welsing, P.
de Jong, A.
Houben, G.F.
Le, T.M.
Publication year
2021
Abstract
Predicting the allergenicity of novel proteins is challenging due to the absence of validated predictive methods and a well-defined reference set of proteins. The prevalence of sensitization could be a parameter to select reference proteins to characterize allergenic proteins. This study investigated whether the prevalence of sensitization of legume extracts and proteins can indeed be used for this purpose. A random sample of suspected food-allergic patients (n=106) was therefore selected. 10 extracts (processed and non-processed) and 18 individual proteins (2S albumins, 7S and 11S globulins) from black lentil, blue and white lupine, chickpea, faba bean, green lentil, pea, peanut, soybean, and white bean were isolated and the prevalence of sensitization and the intensity of IgE binding were evaluated. The prevalence of sensitization ranged from 5.7 % (faba bean and green lentil) to 14.2 % (peanut). The prevalence of sensitization for individual legume proteins ranged from 0.0 % for albumin 1 (pea) to 15.1 %–17.9 % for Ara h 1, 2, 3, and 6 (peanut). The prevalence of sensitization correlated strongly with the intensity of IgE binding for individual proteins (p < 0.05, ρ = 0.894), for extracts no correlation was found. The discovered ranking can be used to select reference proteins for the development and validation of predictive in vitro or in vivo assays for the assessment of the sensitizing potential. © 2021 The Author(s)
Subject
Allergenicity prediction
Allergens
Legumes
Prevalence of sensitization
Ranking of allergens
To reference this document use:
http://resolver.tudelft.nl/uuid:039b1d26-ea6f-4769-8cd0-427683821fbe
DOI
https://doi.org/10.1016/j.toxrep.2021.03.027
TNO identifier
956439
Source
Technical Reports, 8 (8), 767-773
Document type
article